Isothermal titration calorimetry (ITC) is a technique that measures the heat absorbed or released during a binding event. Integrating the raw data leads to the calculation of the enthalpy of binding. A curve is fit with an appropriate model using the data plot of the enthalpy and ligand-to-macromolecule mole ratio. From this curve the thermodynamic parameters, namely the stoichiometry, dissociation constant, and binding entropy, are obtained.
Instrumentation
The VP-capillary DSC is the most sensitive, easy-to-use differential scanning calorimeter to study samples in solution, providing fast, accurate transition midpoint (Tm) determination and ΔH.
TA Instruments Affinity ITC auto
The Affinity ITC Auto is designed for the most challenging life science laboratory environments that require high sensitivity, high productivity and the most advanced ITC technologies.