Differential scanning calorimetry (DSC) is a technique that measures the thermal stability of a macromolecule's tertiary and secondary structure. The raw data obtained in this experiment is a plot of heat capacity difference as a function of temperature, and analysis will yield the macromolecule's Tm as well as the enthalpy of folding.
Instrumentation
MicroCal VP-capillary DSC
The VP-capillary DSC is the most sensitive, easy to use differential scanning calorimeter to study samples in solution, providing fast, accurate transition midpoint (Tm) determination and ΔH.