Patents and Publications

Patents

  1. X. Lian, L.R. Randolph. “Universal Stem Cell and Uses Thereof” – US 18/705,343
  2. X. Lian, Y. Jiang, T. Haideri, A. Howells. “ModRNA-based Cas endonuclease and base editor and uses thereof” PCT/US2023/066305
  3. Lian X, Jiang Y, Randolph LR. “Methods of Generating Hematopoietic Cell Preparations”. PSU 2020-5109 (TPHS Ref. No. 148411.000702)
  4. Lian X, Jiang Y, Guan W, Nouri R. “Nanopore-based detection of analytes”. Penn State Research Foundation Ref. No. 2019-4923
  5. Lian X, Chen C. “GENERATION OF PANCREATIC PROGENITOR CELLS AND BETA CELLS FROM HUMAN PLURIPOTENT STEM CELLS”. Penn State Research Foundation Ref. No. 2018-4800.
  6. Bao X, Lian X, Palecek SP. “Generating epicardium from human pluripotent stem cells.” US Patent Application No. P150192US01. Filed on Feb. 10, 2015.
  7. Lian X, and Chien KR. “Use of Jagged 1/Frizzled 4 as a cell surface marker for isolating human cardiac ventricular progenitor cells” U.S. Provisional Application No. 62/040,892, Filed August 22, 2014.
  8. Lian X, Bao X, and Palecek SP. “Chemically defined albumin-free conditions for cardiomyocyte differentiation of hPSCs” WARF Ref. No. P150011US01, Filed July 17, 2014.
  9. Lian X, Bao X, and Palecek SP. “Simplified methods for generating endothelial cells from human pluripotent stem cells under defined conditions” WARF Ref. No. P130110US02, Filed January 2, 2013.
  10. Lian X, Kamp TJ, and Palecek SP. “Generation of cardiomyocytes from human pluripotent stem cells” WARF Ref. No. P110259WO01, P100142US02/WO01, Filed October 13, 2011, Issued November 2014.
  11. Zhang J, Azarin SM, Lian X, Palecek SP, and Kamp TJ. “Cardiac differentiation of human pluripotent stem cells under defined conditions using matrix overlay methods” US Patent Application No. 12/946019, 2010.

  1. Y Lei, COLLAGEN AND COLLAGEN-ALGINATE HYDROGEL MICROTUBES FOR BIOMANUFACTURING, pending
  2. P Wong, Y Lei, Biosensor Selection of Tumor-Reactive Tumor Infiltrating Lymphocytes, pending
  3. Y Lei, Q Li, O Wang, Dissolvable and degradable artificial circulation systems for large volume tissues, US Patent App. 17/625,686
  4. Y Lei, Large scale cell manufacture system, US Patent App. 17/524,905
  5. CM Fuentes, BL Ekerdt, DV Schaffer, R Segalman, Y Lei, Thermoreversible polymers and methods of use thereof, US Patent 10,982,055
  6. Y Lei, H Viljoen, Cell expansion system, US Patent App. 16/981,050
  7. Y Lei, Personalized cellular biomanufacturing with a closed, miniature cell culture system, US Patent App. 16/462,753

  1. Wang, Y., K Lee. Aptamer-directed enzyme printing for cell encapsulation. Pending
  2. Wang, Y., N Singh. Materials and methods for aptamer selection. WO/2024/118776A1
  3. Wang, Y., Brandon Davis, Peng Shi. Biodegradable DNA-alginate conjugate for reversible protein and cell labeling and imaging. US 2023 0151405A1
  4. Wang, Y., Coyne, J., Kauffman, D., Davis, B. Therapeutic microneedles for heart and hair. PCR/US 2019/048242
  5. Wang, Y., Peng Shi, Synthesis of biomimetic cell wall structure. WO 2020/172590
  6. Wang, Y., Xiaolong Zhang, Nan Zhao. Semi-synthetic tissue constructs for tissue regeneration. US 11,559,606
  7. Wang, Y., Erin Gaddes, Jinping Lai, Niancao Chen. Polymer-based signal amplification for protein and cell detection. US 10,732,175
  8. Wang, Y., Soontornworajit, B, Chen, N. Affinity hydrogel compositions for controlled protein release. PCT/US2011/022128

Publications

  1. Haideri T, Lin J, Bao X*, Lian XL*, MAGIK: A rapid and efficient method to create lineage-specific reporters in human pluripotent stem cells, Stem Cell Reports 19(5):744-757 (2024).
  2. Ream MW, Randolph LN, Jiang Y, Chang Y, Bao X*, Lian XL*, Direct programming of human pluripotent stem cells into endothelial progenitors with SOX17 and FGF2, Stem Cell Reports 19(4):579-595 (2024).
  3. Howells AR, Welch PJ, Kim J, Forest CR, Shi C*, Lian XL*, A drug-selectable acoustic reporter gene system for human cell ultrasound imaging, Bioeng Transl Med 9(2):e10584 (2023).
  4. Chang Y, Cai X, Syahirah R, Yao Y, Xu Y, Jin G, Bhute VJ, Torregrosa-Allen S, Elzey BD, Won YY, Deng Q, Lian XL*, Wang X, Eniola-Adefeso O, Bao X*, CAR-neutrophil mediated delivery of tumor-microenvironment responsive nanodrugs for glioblastoma chemo-immunotherapy, Nat Commun 14(1):2266 (2023).
  5. Tahir H, Howells A, Jiang Y, Yang J, Bao X, Lian XL*, Robust genome editing via modRNA-based Cas9 or base editor in human pluripotent stem cells, Cell Reports Methods 2(9):100290 (2022).
  6. Chang Y, Syahirah R, Wang X, Jin G, Torregrosa-Allen S, Elzey BD, Hummel SN, Wang T, Li C, Lian X*, Deng Q, Broxmeyer HE, Bao X*, Engineering chimeric antigen receptor neutrophils from human pluripotent stem cells for targeted cancer immunotherapy, Cell Reports 40(3):111128 (2022).
  7. Jiang Y, Chen C, Randolph LN, Ye S, Zhang X, Bao X, Lian XL*, Generation of pancreatic progenitors from human pluripotent stem cells by small molecules, Stem Cell Reports 16(9): 2395-2409 (2021).
  8. Foo KS, Lehtinen ML, Leung CY, Lian X, et al., Human ISL1+ Ventricular Progenitors Self-Assemble into an In Vivo Functional Heart Patch and Preserve Cardiac Function Post Infarction, Molecular Therapy, 26(7):1644-1659 (2018).
  9. Bao X, Lian X, Hacker TA, Schmuck EG, Qian T, Bhute VJ, Han T, Shi M, Drowley L, Plowright A, Wang QD, Goumans MJ, and Palecek SP. Long-term self-renewing human epicardial cells generated from pluripotent stem cells under defined xeno-free conditions. Nature Biomedical Engineering 1: 0003 (2017).
  10. Lian X, Bao X, Zilberter M, Westman M, Fisahn A, Hsiao C, Hazeltine LB, Dunn KK, Kamp TJ, Palecek SP. "Chemically defined, albumin-free human cardiomyocyte generation," Nature Methods 12, 595–596 (2015)
  11. Lian X, Bao X, Al-Ahmad A, Liu J, Wu Y, Dong W, Dunn KK, Shusta EV, Palecek SP. “Efficient Differentiation of Human Pluripotent Stem Cells to Endothelial Progenitors via Small-Molecule Activation of WNT Signaling”, Stem Cell Reports 3(5): 804–816 (2014)
  12. Lian X, Zhang J, Azarin SM, Zhu K, Hazeltine LB, Bao X, Hsiao C, Kamp TJ, Palecek SP. “Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/beta-catenin signaling under fully defined conditions”, Nature Protocols 8: 162-175 (2013)
  13. Lian X, Hsiao C, Wilson G, Zhu K, Hazeltine LB, Azarin SM, Raval KK, Zhang J, Kamp TJ, Palecek SP. “Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling”, Proceedings of the National Academy of Sciences 109: E1848-1857 (2012)

  1. Yan Y, Wu X, Pan Y, Wang Y, Lian X, Dong C, Liu W, Wang S, Lei Y. Collagen Hydrogel Tube Microbioreactors for Cell and Tissue Manufacturing. BioRxiv doi.org/10.1101/2025.01.08.631570. 2025 January.
  2. Shi Y, Yang W, Lin H, Han L, Cai AJ, Saraf R, Lei Y, Zhang C. Identification of RNA-based cell-type markers for stem-cell manufacturing systems with a statistical scoring function. Gene Rep. 2024 Mar;34. doi: 10.1016/j.genrep.2023.101869. Epub 2023 Dec 12. PubMed PMID: 38351912; PubMed Central PMCID: PMC10861185.
  3. Liu Q, Liu Z, Gu H, Ge Y, Wu X, Zuo F, Du Q, Lei Y, Wang Z, Lin H. Comparative study of differentiating human pluripotent stem cells into vascular smooth muscle cells in hydrogel-based culture methods. Regen Ther. 2023 Mar;22:39-49. doi: 10.1016/j.reth.2022.12.001. eCollection 2023 Mar. PubMed PMID: 36618488; PubMed Central PMCID: PMC9798140.
  4. Wang Z, Zuo F, Liu Q, Wu X, Du Q, Lei Y, Wu Z, Lin H. Comparative Study of Human Pluripotent Stem Cell-Derived Endothelial Cells in Hydrogel-Based Culture Systems. ACS Omega. 2021 Mar 16;6(10):6942-6952. doi: 10.1021/acsomega.0c06187. eCollection 2021 Mar 16. PubMed PMID: 33748608; PubMed Central PMCID: PMC7970572.
  5. Lin H, Qiu X, Du Q, Li Q, Wang O, Akert L, Wang Z, Anderson D, Liu K, Gu L, Zhang C, Lei Y. Engineered Microenvironment for Manufacturing Human Pluripotent Stem Cell-Derived Vascular Smooth Muscle Cells. Stem Cell Reports. 2019 Jan 8;12(1):84-97. doi: 10.1016/j.stemcr.2018.11.009. Epub 2018 Dec 6. PubMed PMID: 30527760; PubMed Central PMCID: PMC6335449.
  6. Lin H, Li Q, Du Q, Wang O, Wang Z, Akert L, Carlson MA, Zhang C, Subramanian A, Zhang C, Lunning M, Li M, Lei Y. Integrated generation of induced pluripotent stem cells in a low-cost device. Biomaterials. 2019 Jan;189:23-36. doi: 10.1016/j.biomaterials.2018.10.027. Epub 2018 Oct 25. PubMed PMID: 30384126.
  7. Lin H, Du Q, Li Q, Wang O, Wang Z, Liu K, Akert L, Zhang C, Chung S, Duan B, Lei Y. Differentiating human pluripotent stem cells into vascular smooth muscle cells in three dimensional thermoreversible hydrogels. Biomater Sci. 2018 Dec 18;7(1):347-361. doi: 10.1039/c8bm01128a. PubMed PMID: 30483691.
  8. Lin H, Du Q, Li Q, Wang O, Wang Z, Elowsky C, Liu K, Zhang C, Chung S, Duan B, Lei Y. Manufacturing human pluripotent stem cell derived endothelial cells in scalable and cell-friendly microenvironments. Biomater Sci. 2018 Dec 18;7(1):373-388. doi: 10.1039/c8bm01095a. PubMed PMID: 30484784.
  9. Lin H, Du Q, Li Q, Wang O, Wang Z, Liu K, Elowsky C, Zhang C, Lei Y. Hydrogel-Based Bioprocess for Scalable Manufacturing of Human Pluripotent Stem Cell-Derived Neural Stem Cells. ACS Appl Mater Interfaces. 2018 Sep 5;10(35):29238-29250. doi: 10.1021/acsami.8b05780. Epub 2018 Aug 22. PubMed PMID: 30091584.
  10. Lin H, Du Q, Li Q, Wang O, Wang Z, Sahu N, Elowsky C, Liu K, Zhang C, Chung S, Duan B, Lei Y. A Scalable and Efficient Bioprocess for Manufacturing Human Pluripotent Stem Cell-Derived Endothelial Cells. Stem Cell Reports. 2018 Aug 14;11(2):454-469. doi: 10.1016/j.stemcr.2018.07.001. Epub 2018 Aug 2. PubMed PMID: 30078557; PubMed Central PMCID: PMC6092882.
  11. Lin H, Li Q, Wang O, Rauch J, Harm B, Viljoen HJ, Zhang C, Van Wyk E, Zhang C, Lei Y. Automated Expansion of Primary Human T Cells in Scalable and Cell-Friendly Hydrogel Microtubes for Adoptive Immunotherapy. Adv Healthc Mater. 2018 Aug;7(15):e1701297. doi: 10.1002/adhm.201701297. Epub 2018 May 11. PubMed PMID: 29749707.
  12. Ekerdt BL, Fuentes CM, Lei Y, Adil MM, Ramasubramanian A, Segalman RA, Schaffer DV. Thermoreversible Hyaluronic Acid-PNIPAAm Hydrogel Systems for 3D Stem Cell Culture. Adv Healthc Mater. 2018 Jun;7(12):e1800225. doi: 10.1002/adhm.201800225. Epub 2018 May 2. PubMed PMID: 29717823; PubMed Central PMCID: PMC6289514.
  13. Li Q, Lin H, Rauch J, Deleyrolle LP, Reynolds BA, Viljoen HJ, Zhang C, Zhang C, Gu L, Van Wyk E, Lei Y. Scalable Culturing of Primary Human Glioblastoma Tumor-Initiating Cells with a Cell-Friendly Culture System. Sci Rep. 2018 Feb 23;8(1):3531. doi: 10.1038/s41598-018-21927-4. PubMed PMID: 29476107; PubMed Central PMCID: PMC5824878.
  14. Li Q, Lin H, Du Q, Liu K, Wang O, Evans C, Christian H, Zhang C, Lei Y. Scalable and physiologically relevant microenvironments for human pluripotent stem cell expansion and differentiation. Biofabrication. 2018 Feb 1;10(2):025006. doi: 10.1088/1758-5090/aaa6b5. PubMed PMID: 29319535.
  15. Li Q, Wang Q, Wang O, Shao K, Lin H, Lei Y. A simple and scalable hydrogel-based system for culturing protein-producing cells. PLoS One. 2018;13(1):e0190364. doi: 10.1371/journal.pone.0190364. eCollection 2018. PubMed PMID: 29293594; PubMed Central PMCID: PMC5749782.
  16. Lin H, Li Q, Lei Y. An Integrated Miniature Bioprocessing for Personalized Human Induced Pluripotent Stem Cell Expansion and Differentiation into Neural Stem Cells. Sci Rep. 2017 Jan 6;7:40191. doi: 10.1038/srep40191. PubMed PMID: 28057917; PubMed Central PMCID: PMC5216399.
  17. Lin H, Li Q, Lei Y. An Integrated Miniature Bioprocessing for Personalized Human Induced Pluripotent Stem Cell Expansion and Differentiation into Neural Stem Cells. Sci Rep. 2017 Jan 6;7:40191. doi: 10.1038/srep40191. PubMed PMID: 28057917; PubMed Central PMCID: PMC5216399.
  18. Lin H, Li Q, Lei Y. An Integrated Miniature Bioprocessing for Personalized Human Induced Pluripotent Stem Cell Expansion and Differentiation into Neural Stem Cells. Sci Rep. 2017 Jan 6;7:40191. doi: 10.1038/srep40191. PubMed PMID: 28057917; PubMed Central PMCID: PMC5216399.
  19. Lin H, Li Q, Lei Y. An Integrated Miniature Bioprocessing for Personalized Human Induced Pluripotent Stem Cell Expansion and Differentiation into Neural Stem Cells. Sci Rep. 2017 Jan 6;7:40191. doi: 10.1038/srep40191. PubMed PMID: 28057917; PubMed Central PMCID: PMC5216399.

  1. X Wang, B Jia, K Lee, B Davis, C Wen, Y. Wang, H Zheng, Y Wang*, Biomimetic bacterial capsule for enhanced aptamer display and cell recognition. J. Am. Chem. Soc. 2024, 146, 1, 868–877
  2. N Singh, Y Wang, C Wen, B Davis, X Wang, K Lee, Y. Wang *, High-affinity one-step aptamer selection using a non-fouling porous hydrogel. Nature Biotechnology. 2024. 42, 1224-1231
  3. K Lee, B Davis, X Wang, S Mirg, C Wen, L Abune, BE Peterson, L Han, H Chen, H Wang, SE Szczesny, Y Lei, S Kothapalli, Y Wang*, Nanoparticle‐Decorated Biomimetic Extracellular Matrix for Cell Nanoencapsulation and Regulation, Angewandte Chemie International Edition 62 (31), e202306583
  4. P. Shi, Y. Wang *, Synthetic DNA for cell surface engineering (invited), Angewandte Chemie International Edition. 2021, 133 (21), 11684-11695
  5. P. Shi, X. Wang, B. Davis, J. Coyne, C. Dong, Y. Wang *, In situ synthesis of an aptamer-based polyvalent antibody mimic on the cell surface for enhanced interactions between immune and cancer cells. Angewandte Chemie International Edition. 2020, 59 (29), 11892-11897
  6. P. Shi, N. Zhao, J. Coyne, Y. Wang* , DNA-templated synthesis of biomimetic cell wall for nanoencapsulation and protection of live cells. Nature Communications 2019, 10: 2223.
  7. J. Lai, N. Zhao, L. Abune, Y. Wang* , Programmed degradation of hydrogels with a double-locked domain. Angewandte Chemie International Edition. 2019, 58, 2820-2825
  8. Shi, P., Zhao, N., Lai, J., Coyne, J., Gaddes, E.R., Wang, Y.*
  • a. Polyvalent display of biomolecules on live cells
  • b. Angewandte Chemie International Edition. 2018, 57 (23), 6800-6804.
Zhang, Z, Chen, N., Li, S., Battig, M.R., Wang Y.* , Programmable hydrogels for controlled cell catch and release using hybridized aptamers and complementary sequences. Journal of the American Chemical Society. 2012, 134, 15716-15719.Battig, M.R., Soontornworajit, B., Wang Y.* , Programmable release of multiple protein drugs from aptamer-functionalized hydrogels via nucleic acid hybridization. Journal of the American Chemical Society. 2012, 134, 12410-12413.

  1. Sacco, J.S.; Vaneman, Z.T.; Self, A.; Sumner, E.; Kibinda, S.; Sankhe, C.S.; Gomez, E.W. Chemomechanical regulation of EZH2 localization controls epithelial-mesenchymal transition. Journal of Cell Science, 137(22): jcs262190, 2024.
  2. Sankhe, C.S.; Sacco, J.L.; Lawton, J.; Fair, R.; Rezende Soares, D.V.; Aldahdooh, M.K.R.; Gomez, E.D.; Gomez, E.W. Breast cancer cells exhibit mesenchymal-epithelial plasticity following dynamic modulation of matrix stiffness. Advanced Biology, 8(9): 2400087, 2024.
  3. Sacco, J.L., Vaneman, Z.T., Gomez, E.W. Extracellular matrix viscoelasticity regulates TGFβ1-induced epithelial-mesenchymal transition and apoptosis via integrin linked kinase. Journal of Cellular Physiology, 239(2): e31165, 2024.
  4. Nalluri, S.M.; Sankhe, C.S.; O’Connor, J.W.; Blanchard, P.L.; Khouri, J.N.; Phan, S.H.; Virgi, G.; Gomez, E.W. Crosstalk between ERK and MRTF-A signaling regulates TGFβ1-induced epithelial mesenchymal transition. Journal of Cellular Physiology, 237(5): 2503-2515, 2022.
  5. O’Connor, J.W., Mistry, K., Detweiler, D., Wang, C., Gomez, E.W. Cell-cell contact and matrix adhesion promote αSMA expression during TGFβ1-induced epithelial-myofibroblast transition via Notch and MRTF-A. Scientific Reports, 6, 26226, 2016.
  6. O’Connor, J.W., Riley, P.N., Nalluri, S.M., Ashar, P.K., Gomez, E.W. Matrix rigidity mediates TGFβ1-induced epithelial-myofibroblast transition by controlling cytoskeletal organization and MRTF-A localization. Journal of Cellular Physiology, 230, 1829-1839, 2015.