Scott Showalter

Professor of Chemistry; Professor of Biochemistry and Molecular Biology

Scott Showalter

Research Summary

Biophysical Chemistry applied to solution NMR spectroscopy of partially disordered proteins. NMR studies of protein dynamics coupled with computational and theoretical studies of the coupling between nuclear spin relaxation and molecular motion.

Huck Affiliations

Links

Most Recent Publications

Molecular insights into the interaction between a disordered protein and a folded RNA

R Mitra, E Usher, S Dedeoğlu, Selin Dedeoǧlu, M Crotteau, O Fraser, Neela H. Yennawar, V Gadkari, B Ruotolo, A Holehouse, L Salmon, Scott A. Showalter, Bardwell JCA, James C.A. Bardwell, 2024, Proceedings of the National Academy of Sciences of the United States of America on p. e2409139121

From molecular descriptions to cellular functions of intrinsically disordered protein regions

Wei Chen, Olivia A. Fraser, Christy George, Scott A. Showalter, 2024, Biophysics Reviews

Acetylation-Dependent Compaction of the Histone H4 Tail Ensemble

Sophia M. Dewing, Tien M. Phan, Emma J. Kraft, Jeetain Mittal, Scott A. Showalter, 2024, Journal of Physical Chemistry B on p. 10636-10649

Advances in direct detection of lysine methylation and acetylation by nuclear magnetic resonance using <sup>13</sup>C-enriched cofactors

Olivia A. Fraser, Kevin E.W. Namitz, Scott A. Showalter, 2023, ImmunoMethods on p. 72-83

Phase separation promotes a highly active oligomeric scaffold of the MLL1 core complex for regulation of histone H3K4 methylation

Kevin E.W. Namitz, Scott A. Showalter, Michael S. Cosgrove, 2023, Journal of Biological Chemistry

A direct nuclear magnetic resonance method to investigate lysine acetylation of intrinsically disordered proteins

Olivia A. Fraser, Sophia M. Dewing, Emery T. Usher, Christy George, Scott A. Showalter, 2023, Frontiers in Molecular Biosciences

Biophysical insights into glucose-dependent transcriptional regulation by PDX1

Emery T. Usher, Scott A. Showalter, 2022, Journal of Biological Chemistry

Probing multiple enzymatic methylation events in real time with NMR spectroscopy

Emery T. Usher, Kevin E.W. Namitz, Michael S. Cosgrove, Scott A. Showalter, 2021, Biophysical Journal on p. 4710-4721

Transient Electrostatic Interactions between Fcp1 and Rap74 Bias the Conformational Ensemble of the Complex with Minimal Impact on Binding Affinity

Victor A. Prieto, Kevin E.W. Namitz, Scott A. Showalter, 2021, Journal of Physical Chemistry B on p. 10917-10927

Intrinsically disordered substrates dictate SPOP subnuclear localization and ubiquitination activity

Emery T. Usher, Nafiseh Sabri, Roman Rohac, Amie K. Boal, Tanja Mittag, Scott A. Showalter, 2021, Journal of Biological Chemistry

Most-Cited Papers

Structural Basis for Rare Earth Element Recognition by Methylobacterium extorquens Lanmodulin

Erik C. Cook, Emily R. Featherston, Scott A. Showalter, Joseph A. Cotruvo, 2019, Biochemistry on p. 120-125

Application of NMR to studies of intrinsically disordered proteins

Eric B. Gibbs, Erik C. Cook, Scott A. Showalter, 2017, Archives of Biochemistry and Biophysics on p. 57-70

Phosphorylation induces sequence-specific conformational switches in the RNA polymerase II C-terminal domain

E Gibbs, F Lu, B Portz, M Fisher, B Medellin, Tatiana Laremore, Y Zhang, David S. Gilmour, Scott A. Showalter, 2017, Nature Communications on p. 15233

Structural heterogeneity in the intrinsically disordered RNA polymerase II C-terminal domain

Bede Portz, Feiyue Lu, Eric B. Gibbs, Joshua E. Mayfield, M. Rachel Mehaffey, Yan Jessie Zhang, Jennifer S. Brodbelt, Scott A. Showalter, David S. Gilmour, 2017, Nature Communications

Quantitative biophysical characterization of intrinsically disordered proteins

Eric B. Gibbs, Scott A. Showalter, 2015, Biochemistry on p. 1314-1326

A primer for carbon-detected NMR applications to intrinsically disordered proteins in solution

Monique Bastidas, Eric B. Gibbs, Debashish Sahu, Scott A. Showalter, 2015, Concepts in Magnetic Resonance Part A: Bridging Education and Research on p. 54-66

Ultrasound-Guided Cytosolic Protein Delivery via Transient Fluorous Masks

Janna Sloand, Janna Sloand^, Eric Cook, Theodore Nguyen, Scott A. Zinck, Tawanda Zimudzi, Erik C. Cook, Scott Zinck, S Showalter, Tawanda J. Zimudzi, Scott Schowalter, Scott A. Showalter, Julianna Simon, S Medina, Adam B. Glick, Julianna Simon, Scott Medina*, 2020, ACS Nano on p. 4061-4073

The Use of <sup>13</sup>C Direct-Detect NMR to Characterize Flexible and Disordered Proteins

Erik C. Cook, Grace A. Usher, Scott A. Showalter, 2018, on p. 81-100

An adjunctive therapy administered with an antibiotic prevents enrichment of antibiotic-resistant clones of a colonizing opportunistic pathogen

Valerie J. Morley, Clare Kinnear, Derek G. Sim, Samantha N. Olson, Samantha Olsen, Lindsey Jackson, Elsa Hansen, Grace Usher, Scott A. Showalter, Manjunath Pai, Robert Woods, Andrew F. Read, 2020, eLife on p. 1-17

Assessing Coupled Protein Folding and Binding Through Temperature-Dependent Isothermal Titration Calorimetry

Debashish Sahu, Monique Bastidas, Chad W. Lawrence, William G. Noid, Scott A. Showalter, 2016, Methods in Enzymology on p. 23-45