Mark Hedglin
Assistant Professor of Chemistry; Assistant Professor of Biochemistry and Molecular Biology
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210A Wartik Lab
University Park, PA 16802 - muh218@psu.edu
- 814-863-1080
Research Summary
Deciphering how efficient and faithful replication of the human genome is achieved within the highly-complex, dynamic, and reactive environment of the nucleus. Identifying pathways for genomic instability in humans, identifying novel oncogenic drug targets, developing better chemotherapeutic treatments for human cancers caused by genomic instability.
Huck Graduate Students
Huck Affiliations
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Most Recent Publications
PARP2 promotes Break Induced Replication-mediated telomere fragility in response to replication stress
Daniela Muoio, Natalie Laspata, Rachel L. Dannenberg, Caroline Curry, Simone Darkoa-Larbi, Mark Hedglin, Shikhar Uttam, Elise Fouquerel, 2024, Nature Communications
Direct, ensemble FRET approaches to monitor transient state kinetics of human DNA polymerase delta holoenzyme assembly and initiation of DNA synthesis
Jessica Norris, Mark Hedglin, 2024, Methods in Enzymology
Replication protein A dynamically re-organizes on primer/template junctions to permit DNA polymerase δ holoenzyme assembly and initiation of DNA synthesis
Jessica L. Norris, Lindsey O. Rogers, Kara G. Pytko, Rachel L. Dannenberg, Samuel Perreault, Vikas Kaushik, Sahiti Kuppa, Edwin Antony, Mark Hedglin, 2024, Nucleic Acids Research on p. 7650-7664
Replication of [AT/TA]<sub>25</sub> Microsatellite Sequences by Human DNA Polymerase δ Holoenzymes Is Dependent on dNTP and RPA Levels
Kara G. Pytko, Rachel L. Dannenberg, Kristin A. Eckert, Mark Hedglin, 2024, Biochemistry
Stepwise requirements for polymerases δ and θ in theta-mediated end joining
Susanna Stroik, Juan Carvajal-Garcia, Dipika Gupta, Alyssa Edwards, Adam Luthman, David W. Wyatt, Rachel L. Dannenberg, Wanjuan Feng, Thomas A. Kunkel, Gaorav P. Gupta, Mark Hedglin, Richard Wood, Sylvie Doublié, Eli Rothenberg, Dale A. Ramsden, 2023, Nature on p. 836-841
Mechanistic insight into AP-endonuclease 1 cleavage of abasic sites at stalled replication fork mimics
Nicole M. Hoitsma, Jessica Norris, Thu H. Khoang, Vikas Kaushik, Rahul Chadda, Edwin Antony, Mark Hedglin, Bret D. Freudenthal, 2023, Nucleic Acids Research on p. 6738-6753
Tracking of progressing human DNA polymerase δ holoenzymes reveals distributions of DNA lesion bypass activities
Rachel L. Dannenberg, Joseph A. Cardina, Kara G. Pytko, Mark Hedglin, 2022, Nucleic Acids Research on p. 9893-9908
Structure of the human clamp loader reveals an autoinhibited conformation of a substrate-bound AAA+ switch
Christl Gaubitz, Xingchen Liu, Joseph Magrino, Nicholas P. Stone, Jacob Landeck, Mark Hedglin, Brian A. Kelch, 2020, Proceedings of the National Academy of Sciences of the United States of America on p. 23571-23580
Structural basis of reiterative transcription from the pyrG and pyrBI promoters by bacterial RNA polymerase
Yeonoh Shin, Mark Hedglin, Katsuhiko S. Murakami, 2020, Nucleic Acids Research on p. 2144-2155
Rad6/Rad18 Competes with DNA Polymerases ν and δfor PCNA Encircling DNA
Mingjie Li, Leah Larsen, Mark Hedglin, 2020, Biochemistry on p. 407-416
Most-Cited Papers
Regulation of Rad6/Rad18 Activity during DNA Damage Tolerance
Mark Hedglin, Stephen J. Benkovic, 2015, Annual Review of Biophysics and Biomolecular Structure on p. 207-228
Stability of the human polymerase δ holoenzyme and its implications in lagging strand DNA synthesis
Mark Hedglin, Binod Pandey, Stephen J. Benkovic, 2016, Proceedings of the National Academy of Sciences of the United States of America on p. E1777-E1786
Eukaryotic Translesion DNA Synthesis on the Leading and Lagging Strands: Unique Detours around the Same Obstacle
Mark Hedglin, Stephen J. Benkovic, 2017, Chemical Reviews on p. 7857-7877
Characterization of human translesion DNA synthesis across a UV-induced DNA lesion
Mark Hedglin, Binod Pandey, Stephen J. Benkovic, 2016, eLife
Structure of the human clamp loader reveals an autoinhibited conformation of a substrate-bound AAA+ switch
Christl Gaubitz, Xingchen Liu, Joseph Magrino, Nicholas P. Stone, Jacob Landeck, Mark Hedglin, Brian A. Kelch, 2020, Proceedings of the National Academy of Sciences of the United States of America on p. 23571-23580
Replication protein A dynamically regulates monoubiquitination of proliferating cell nuclear antigen
Mark Hedglin, Mahesh Aitha, Anthony Pedley, Stephen Benkovic, 2019, Journal of Biological Chemistry on p. 5157-5168
Replication Protein A Prohibits Diffusion of the PCNA Sliding Clamp along Single-Stranded DNA
Mark Hedglin, Stephen J. Benkovic, 2017, Biochemistry on p. 1824-1835
Probing the DNA structural requirements for facilitated diffusion
Mark Hedglin, Yaru Zhang, Patrick J. O'Brien, 2015, Biochemistry on p. 557-566
Monitoring the Retention of Human Proliferating Cell Nuclear Antigen at Primer/Template Junctions by Proteins That Bind Single-Stranded DNA
Mark Hedglin, Mahesh Aitha, Stephen J. Benkovic, 2017, Biochemistry on p. 3415-3421
PCNA Monoubiquitination Is Regulated by Diffusion of Rad6/Rad18 Complexes along RPA Filaments
Mingjie Li, Bhaswati Sengupta, Stephen J. Benkovic, Tae Hee Lee, Mark Hedglin, 2020, Biochemistry on p. 4694-4702