Gallery

Gallery of structures produced using PSU Cryo-EM facility

Apoferritin: 1.97 Å (Krios)

Apoferritin: 1.97 Å (Krios)

Cryo-EM map of apoferritin at 1.97 Å resolution. Data collected on an in-house Thermo Fisher Titan Krios electron microscope (300 kV) with spherical aberration correction, using a Falcon 4EC direct detection camera.

Intranasal SARS-CoV-2 RBD decorated nanoparticle vaccine

Nanocage Protein

Intranasal SARS-CoV-2 RBD decorated nanoparticle vaccine enhances viral clearance in the Syrian hamster model.

Patel DR, Minns AM, Sims DG, Field CJ, Kerr AE, Heinly T, Luley EH, Rossi RM, Bator C, Mostafa IM, Hafenstein SL, Lindner SE, Sutton TC. bioRxiv. 2022 Dec 14:2022.10.27.514054. doi: 10.1101/2022.10.27.514054. Preprint.

  • PDB:8E01 (3.4 Å)
  • EMD-27812
Cryo-EM Structure of Gokushovirus ΦEC6098

Gokushovirus

Cryo-EM Structure of Gokushovirus ΦEC6098 Reveals a Novel Capsid Architecture for a Single-Scaffolding Protein, Microvirus Assembly System.

Lee H, Baxter AJ, Bator CM, Fane BA, Hafenstein SL.

J Virol. 2022 Nov 9;96(21):e0099022. doi: 10.1128/jvi.00990-22. Epub 2022 Oct 18.

  • PDB:8DES (2.6 Å)
  • EMD-27397
Authentic Murine Papillomavirus Capsids

Murine Papillomavirus

Cryo EM Analysis Reveals Inherent Flexibility of Authentic Murine Papillomavirus Capsids.

Hartmann SR, Goetschius DJ, Hu J, Graff JJ, Bator CM, Christensen ND, Hafenstein SL.

Viruses. 2021 Oct 7;13(10):2023. doi: 10.3390/v13102023.

  • PDB:7YRJ (3.3 Å)
  • EMD-24741
High-resolution asymmetric structure of a Fab-virus complex

Canine Parvovirus in Complex with Fab 14

High-resolution asymmetric structure of a Fab-virus complex reveals overlap with the receptor binding site.

Goetschius DJ, Hartmann SR, Organtini LJ, Callaway H, Huang K, Bator CM, Ashley RE, Makhov AM, Conway JF, Parrish CR, Hafenstein SL.

Proc Natl Acad Sci U S A. 2021 Jun 8;118(23):e2025452118. doi: 10.1073/pnas.2025452118.

High resolution cryo EM analysis of HPV16

Human Papillomavirus 16 (HPV16)

High resolution cryo EM analysis of HPV16 identifies minor structural protein L2 and describes capsid flexibility.

Goetschius DJ, Hartmann SR, Subramanian S, Bator CM, Christensen ND, Hafenstein SL.

Sci Rep. 2021 Feb 10;11(1):3498. doi: 10.1038/s41598-021-83076-5.

  • PDB: 7KZF (3.1 Å)
  • EMD-23081
Identification of a pocket factor that is critical to Zika virus assembly

Zika Virus

Identification of a pocket factor that is critical to Zika virus assembly.

DiNunno NM, Goetschius DJ, Narayanan A, Majowicz SA, Moustafa I, Bator CM, Hafenstein SL, Jose J.

Nat Commun. 2020 Oct 2;11(1):4953. doi: 10.1038/s41467-020-18747-4.

  • PDB:7JyI (4.0 Å)
  • EMD-22526
  • EMD-22527
Antibody escape by polyomavirus capsid mutation facilitates neurovirulence

Mouse Polyomavirus (MuPyV) in Complex with Fab

Antibody escape by polyomavirus capsid mutation facilitates neurovirulence.

Lauver MD, Goetschius DJ, Netherby-Winslow CS, Ayers KN, Jin G, Haas DG, Frost EL, Cho SH, Bator CM, Bywaters SM, Christensen ND, Hafenstein SL, Lukacher AE.

Elife. 2020 Sep 17;9:e61056. doi: 10.7554/eLife.61056.

  • EMDB-22645 and EMDB-22646
  • PDB 7K24, 7K25; EMDB 22642, 22643
  • PDB 7K22, 7K23, EMDB 22640, 22641
Cryo-EM structure of catalytic ribonucleoprotein complex RNase MRP

RNase MRP Homoenzyme Complex

Cryo-EM structure of catalytic ribonucleoprotein complex RNase MRP.

Perederina A, Li D, Lee H, Bator C, Berezin I, Hafenstein SL, Krasilnikov AS.

Nat Commun. 2020 Jul 10;11(1):3474. doi: 10.1038/s41467-020-17308-z.

  • PDB: 6W6V (3.0 Å)
  • EMD-21564
Scaffold Simplification Strategy Leads to a Novel Generation of Dual Human Immunodeficiency Virus and Enterovirus-A71 Entry Inhibitors

Enterovirus A71 (EV-A71) in Complex with a Potent Inhibitor

Scaffold Simplification Strategy Leads to a Novel Generation of Dual Human Immunodeficiency Virus and Enterovirus-A71 Entry Inhibitors.

Martínez-Gualda B, Sun L, Martí-Marí O, Noppen S, Abdelnabi R, Bator CM, Quesada E, Delang L, Mirabelli C, Lee H, Schols D, Neyts J, Hafenstein S, Camarasa MJ, Gago F, San-Félix A.

J Med Chem. 2020 Jan 9;63(1):349-368. doi: 10.1021/acs.jmedchem.9b01737. Epub 2019 Dec 20.

  • PDB: 6UH1, 6HU6, 6UH7
  • EMD-20766
  • EMD-20768
  • EMD-20769
Transferrin receptor binds virus capsid with dynamic motion

Canine Parvovirus (CPV) in Complex with Transferrin Receptor Type-1 (TfR)

Transferrin receptor binds virus capsid with dynamic motion.

Lee H, Callaway HM, Cifuente JO, Bator CM, Parrish CR, Hafenstein SL.Proc Natl Acad Sci U S A. 2019 Oct 8;116(41):20462-20471. doi: 10.1073/pnas.1904918116. Epub 2019 Sep 23.

Filling Adeno-Associated Virus Capsids: Estimating Success by Cryo-Electron Microscopy

Adeno-Associated Virus (AAV) Capsids

Filling Adeno-Associated Virus Capsids: Estimating Success by Cryo-Electron Microscopy.

Subramanian S, Maurer AC, Bator CM, Makhov AM, Conway JF, Turner KB, Marden JH, Vandenberghe LH, Hafenstein SL.Hum Gene Ther. 2019 Dec;30(12):1449-1460. doi: 10.1089/hum.2019.041. Epub 2019 Nov 14.

  • EMD-20625
  • EMD-20624 (3.2 Å, 3.4 Å)
Viral engagement with host receptors blocked by a novel class of tryptophan dendrimers that targets the 5-fold-axis of the enterovirus-A71 capsid

Enterovirus A71 (EV-A71) in Complex with MADAL385 Inhibitor

Viral engagement with host receptors blocked by a novel class of tryptophan dendrimers that targets the 5-fold-axis of the enterovirus-A71 capsid.

Sun L, Lee H, Thibaut HJ, Lanko K, Rivero-Buceta E, Bator C, Martinez-Gualda B, Dallmeier K, Delang L, Leyssen P, Gago F, San-Félix A, Hafenstein S, Mirabelli C, Neyts J.

PLoS Pathog. 2019 May 9;15(5):e1007760. doi: 10.1371/journal.ppat.1007760. eCollection 2019 May.PMID: 31071193 Free PMC article.

  • PDB: 6DIJ, 6DIZ
  • EMD-7905
  • EMD-7913
Allosteric mechanism of transcription inhibition by NusG-dependent pausing of RNA polymerase

Paused Transcription Complex (PTC) and Transcription Elongation Complex (TEC) of Bacterial RNAP

Allosteric mechanism of transcription inhibition by NusG-dependent pausing of RNA polymerase.

Vishwakarma RK, Qayyum MZ, Babitzke P, Murakami KS.

Proc Natl Acad Sci U S A. 2023 Feb 14;120(7):e2218516120. doi: 10.1073/pnas.2218516120. Epub 2023 Feb 6.

Cryo-EM structure of the human Sirtuin 6-nucleosome complex

Human Sirtuin 6-nucleosome Complex

Chio, U.s, O. Rechiche, A.R. Bryll, J. Zhu, J.L. Feldman, E.M. Leith, C.L Peterson, S. Tan, J.-P. Armache (2023) Cryo-EM structure of the human Sirtuin 6-nucleosome complex,

Science Advances, 9:eadf7586.

  • PDB: 8G57 (3.1 Å)
  • EMD-29735