Global metabolomics and lipidomics
Uses
- Global profiling of metabolites and lipids
- Compound identification
- Isotope-tracing
Specifications
- Quadrupole mass filter, linear ion trap, and orbitrap mass analyzers
- Resolving power: up to 1,000,000 FWHM (full width at half maximum), with isotopic fidelity up to 240,000 FWHM at m/z 200
- Synchronous precursor selection for MS and MSn (up to 10) experiments
- Multiple fragmentation techniques: CID (collision-induced dissociation), HCD (high energy collisional dissociation) available at any stage of MSn with detection in either orbitrap or LIT
- Acquisition rates: up to 30 Hz for Orbitrap and 40 Hz for LIT MSn analyses
- Mass accuracy: <3 ppm RMS using external calibration, <1 ppm RMS using internal calibration
- Mass range: m/z 50-2,000 or 200-4,000 or up to 6,000 without precursor ion selection
- Polarity switching: <1.1 sec (one full scan positive mode and one full scan negative mode at resolution setting of 30,000)
Dynamic range: >5,000 within a single OTMS spectrum
Details
Please contact the Metabolomics Core Director, Ashley Shay, for more information.