Agilent TapeStation 4150

Specification

• 15 samples plus a ladder can be assayed on a single RNA or DNA Tape
• An objective measure of RNA quality, the RNA Integrity Number (RIN) can be determined for total RNA samples run on either the RNA or RNA HS Tape
• The RIN of total RNA samples at concentrations of 1 ng/ul – 25ng/ul can be determined on RNA HS Tapes
• The RIN of total RNA samples at concentrations of 25 ng/ul – 500 ng/ul can be analyzed on RNA Tapes
• NGS libraries or fragmented DNA samples from 100 nt – 5000 nt at concentrations of 0.1 ng/ul – 50 ng/ul can be analyzed on the DNA 5000 Tape. The DNA 5000 HS Tape can analyze fragments from 100 – 5000 nt at concentrations of 10 pg/ul – 1000 pg/ul
• NGS libraries or fragmented DNA from 35 nt – 1000 nt and concentrations of 0.1 ng/ul – 50/ng/ul can be analyzed on the DNA 1000 Tape. The DNA 1000 HS Tape can analyze fragments from 35 – 1000 nt at concentrations of 10 – 1000 pg/ul
• An objective measure of DNA quality, the DNA Integrity Number (DIN) can be determined for genomic DNA samples run on a Genomic DNA Tape
• The Genomic DNA Tape can analyze genomic DNA from 200 nt – 60,000 nt at concentrations from 5 – 300 ng/ul

Details

• The Tape will consume 1-2 ul of sample. Please provide us a minimum of 5 ul per sample. This allows us to check the concentration of your sample prior to assaying it on the TapeStation. This also provides enough sample it can be re-run if necessary. We are happy to return the unused portion.
• Provide your samples in standard 0.5 or 1.5 ml microfuge tubes. No 0.2 ml PCR tubes or PCR strip tubes allowed.

Used for a variety of applications:

• NGS library QC
• Total RNA RIN determination 
• Genomic DNA DIN determination
• Accurate size determination and quantification of DNA fragments

This instrument is operated by Genomics Core Facility staff in conjunction with next-generation sequencing services. 

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