Circular dichroism (CD) spectroscopy is a powerful technique for probing the secondary and tertiary structures of proteins, peptides, and other chiral biomolecules in solution.
By measuring the differential absorption of left- and right-circularly polarized light, CD provides insights into the conformational state, folding, stability, and structural changes of macromolecules under varying environmental conditions such as temperature, pH, or ligand binding. It is particularly well-suited for characterizing alpha-helices, beta-sheets, and random coil content, and can be used to monitor folding kinetics or assess the effects of mutations or buffer formulations. CD is a rapid, label-free, and non-destructive method ideal for both routine and advanced structural studies.
Instrumentation
Jasco J-1500 Circular Dichroism (CD) Spectrometer